Extensive 3′ alternative splicing of the mu opioid receptor gene
Jin Xu, Zhigang Lu, Ankita Narayan, Valerie P. Le Rouzic, Mingming Xu, Amanda Hunkele, Taylor G. Brown, William F. Hoefer, Grace C. Rossi, Richard C. Rice, Arlene Martínez-Rivera, Anjali M. Rajadhyaksha, Luca Cartegni, Daniel L. Bassoni, Gavril W. Pasternak, Ying-Xian Pan
Effect of the truncation of exon 7–encoded C-terminal tails on morphine-induced receptor desensitization by morphine-stimulated [35S]GTPγS binding in the hypothalamus and brain stem.
Morphine tolerance was induced in mE7M-B6 and mE7M-129 mouse models by the same paradigms used for Figure 1. A control group injected with saline was also included for both WT and homozygous mice. [35S]GTPγS-binding assay was performed using membranes prepared from 6 regions that were dissected after the last morphine injection on day 5, as described in Supplemental Methods. Percentage of maximal stimulation (over basal level) and EC50 values were calculated by nonlinear regression analysis (Prism). Only percentage of maximal stimulation in the hypothalamus and brain stem in mE7M-B6 and mE7M-129 mice are shown. The EC50 and percentage of maximal stimulation of all the regions among WT and homozygous mice treated with saline or morphine are listed in Table 5 and dose-response curves in Supplemental Figure 8. The results are shown as mean ± SEM of percentage of maximal stimulation values determined from 3 to 4 independent experiments. *