[HTML][HTML] Bafilomycin A1 treatment retards transferrin receptor recycling more than bulk membrane recycling
Treatment of Chinese hamster ovary cells with the vacuolar proton pump inhibitor
bafilomycin A 1 causes a 2-fold retardation in the rate of recycling of transfected human
transferrin receptors back to the cell surface as measured using biochemical assays
(Johnson, LS, Dunn, KW, Pytowski, B., and McGraw, TE (1993) Mol. Biol. Cell 4, 1251–
1266). We have used quantitative fluorescence microscopy to determine which step (s) in
the endocytic recycling pathway are affected. We show that removal of transferrin from …
bafilomycin A 1 causes a 2-fold retardation in the rate of recycling of transfected human
transferrin receptors back to the cell surface as measured using biochemical assays
(Johnson, LS, Dunn, KW, Pytowski, B., and McGraw, TE (1993) Mol. Biol. Cell 4, 1251–
1266). We have used quantitative fluorescence microscopy to determine which step (s) in
the endocytic recycling pathway are affected. We show that removal of transferrin from …
