Nitric oxide synthase: irreversible inhibition by L-NG-nitroarginine in brain in vitro and in vivo
MA Dwyer, DS Bredt, SH Snyder - Biochemical and biophysical research …, 1991 - Elsevier
MA Dwyer, DS Bredt, SH Snyder
Biochemical and biophysical research communications, 1991•ElsevierInhibition of nitric oxide (NO) synthase activity by LN G-Nitroarginine (NO 2 Arg) in brain
preparations is not reversed by dialysis and is enhanced by prolonged preincubation of NO
2 Arg with the enzyme. By contrast, the weaker inhibition by NO 2 Arg of macrophage NO
synthase is fully reversible. NO 2 Arg inhibits NO synthase activity in the brain after ip
administration of 5 or 50 mg/kg. This in vivo inhibition also appears to be irreversible. The
potent in vivo inhibition of central NO synthase by NO 2 Arg may facilitate studies of the …
preparations is not reversed by dialysis and is enhanced by prolonged preincubation of NO
2 Arg with the enzyme. By contrast, the weaker inhibition by NO 2 Arg of macrophage NO
synthase is fully reversible. NO 2 Arg inhibits NO synthase activity in the brain after ip
administration of 5 or 50 mg/kg. This in vivo inhibition also appears to be irreversible. The
potent in vivo inhibition of central NO synthase by NO 2 Arg may facilitate studies of the …
Inhibition of nitric oxide (NO) synthase activity by LN G-Nitroarginine (NO 2 Arg) in brain preparations is not reversed by dialysis and is enhanced by prolonged preincubation of NO 2 Arg with the enzyme. By contrast, the weaker inhibition by NO 2 Arg of macrophage NO synthase is fully reversible. NO 2 Arg inhibits NO synthase activity in the brain after ip administration of 5 or 50 mg/kg. This in vivo inhibition also appears to be irreversible. The potent in vivo inhibition of central NO synthase by NO 2 Arg may facilitate studies of the physiologic function of NO as a neuronal messenger.
Elsevier
