[PDF][PDF] Demonstration of the Th1 to Th2 cytokine shift during the course of HIV-1 infection using cytoplasmic cytokine detection on single cell level by flow cytometry
SA Klein, JM Dobmeyer, TS Dobmeyer, M Pape… - … SCIENCE THEN RAPID …, 1997 - Citeseer
Objective: To characterize changes of Th1/Th2 cytokine production by peripheral blood
mononuclear cells (PBMC) that occur during the course of HIV infection by cytoplasmic
cytokine staining on single cell level. Design and methods: Mitogen-stimulated PBMC from
16 healthy donors, 18 HIV-1-infected individuals without AIDS and 14 patients with AIDS
were stained intracellularly with fluorescein-labelled MAb against interleukin (IL)-2, IL-4, IL-
10 and interferon (IFN)-γ. Additionally, co-staining of CD4+ T-cell, CD8+ T-cell, natural killer …
mononuclear cells (PBMC) that occur during the course of HIV infection by cytoplasmic
cytokine staining on single cell level. Design and methods: Mitogen-stimulated PBMC from
16 healthy donors, 18 HIV-1-infected individuals without AIDS and 14 patients with AIDS
were stained intracellularly with fluorescein-labelled MAb against interleukin (IL)-2, IL-4, IL-
10 and interferon (IFN)-γ. Additionally, co-staining of CD4+ T-cell, CD8+ T-cell, natural killer …