[CITATION][C] Transport of glucose across the lens surfaces
JE Harris, JD Hauschildt, LT Nordquist - American Journal of …, 1955 - Elsevier
JE Harris, JD Hauschildt, LT Nordquist
American Journal of Ophthalmology, 1955•ElsevierMETHODS Rabbit lenses were used throughout. These were removed by a posterior ap
proach in a manner previously described. 3 Generally, two lenses from different animals
were incubated at 37 C. in a small Erlenmeyer flask containing 10 ml. per lens of a modified
Tyrode's solution3 to which extra glucose was added. The media was equilibrated with a 95
per cent O2-5.0 percent C02 mixture, except when anaerobic conditions were used. Un less
otherwise specified the glucose concen tration employed was 400 mg. percent and the …
proach in a manner previously described. 3 Generally, two lenses from different animals
were incubated at 37 C. in a small Erlenmeyer flask containing 10 ml. per lens of a modified
Tyrode's solution3 to which extra glucose was added. The media was equilibrated with a 95
per cent O2-5.0 percent C02 mixture, except when anaerobic conditions were used. Un less
otherwise specified the glucose concen tration employed was 400 mg. percent and the …
METHODS
Rabbit lenses were used throughout. These were removed by a posterior ap proach in a manner previously described. 3 Generally, two lenses from different animals were incubated at 37 C. in a small Erlenmeyer flask containing 10 ml. per lens of a modified Tyrode's solution3 to which extra glucose was added.
The media was equilibrated with a 95 per cent O2-5.0 percent C02 mixture, except when anaerobic conditions were used. Un less otherwise specified the glucose concen tration employed was 400 mg. percent and the period of incubation six hours. Under these circumstances the glucose concentra tion of the bathing medium remained sub stantially constant under all experimental conditions.
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