Demonstration of the interaction of thioredoxin with p40phox, a phagocyte oxidase component, using a yeast two-hybrid system

A Nishiyama, T Ohno, S Iwata, M Matsui, K Hirota… - Immunology letters, 1999 - Elsevier
Thioredoxin (TRX) has disulfide reducing activity and is reported to be involved in various
cellular functions including the promotion of cell growth and apoptosis. To help understand
the molecular mechanism through which TRX is involved in immunological systems, we
screened a cDNA library derived from a B-cell population of Epstein–Barr virus-transformed
human peripheral blood lymhocyte for TRX binding proteins by use of a yeast two-hybrid
system. Among plasmids from positive clones, a plasmid contained an insert which has …