Initial deactivation of florisil adorbent for column chromatographic separation of lipids
T Todoroki, K Imai, K Matsumoto, S Kano - Analyst, 1983 - pubs.rsc.org
T Todoroki, K Imai, K Matsumoto, S Kano
Analyst, 1983•pubs.rsc.orgFlorisil column chromatography has been used extensively for the separation of lipids, l but
its high adsorptive activity often resulted in low recoveries of lipids and low reproducibility.
Therefore, to lower the activity and keep its activity constant, pre-conditioning of Florisil with
50% V/V methanol solution was performed, and better resolution and recoveries for all
classes of lipids were obtained, as described in this paper.
its high adsorptive activity often resulted in low recoveries of lipids and low reproducibility.
Therefore, to lower the activity and keep its activity constant, pre-conditioning of Florisil with
50% V/V methanol solution was performed, and better resolution and recoveries for all
classes of lipids were obtained, as described in this paper.
Florisil column chromatography has been used extensively for the separation of lipids, l but its high adsorptive activity often resulted in low recoveries of lipids and low reproducibility. Therefore, to lower the activity and keep its activity constant, pre-conditioning of Florisil with 50% V/V methanol solution was performed, and better resolution and recoveries for all classes of lipids were obtained, as described in this paper.
The Royal Society of Chemistry