[HTML][HTML] β-Amyloid induces paired helical filament-like tau filaments in tissue culture
A Ferrari, F Hoerndli, T Baechi, RM Nitsch… - Journal of Biological …, 2003 - ASBMB
Paired helical filaments (PHF) are the principal pathologic components of neurofibrillary
tangles in Alzheimer's disease (AD). To reproduce the formation of PHF in tissue culture, we
stably expressed human tau with and without pathogenic mutations in human SH-SY5Y
cells and exposed them for 5 days to aggregated synthetic β-amyloid peptide (Aβ 42). This
caused a decreased solubility of tau along with the generation of PHF-like tau-containing
filaments. These were 20 nm wide and had periodicities of 130-140 nm in the presence of …
tangles in Alzheimer's disease (AD). To reproduce the formation of PHF in tissue culture, we
stably expressed human tau with and without pathogenic mutations in human SH-SY5Y
cells and exposed them for 5 days to aggregated synthetic β-amyloid peptide (Aβ 42). This
caused a decreased solubility of tau along with the generation of PHF-like tau-containing
filaments. These were 20 nm wide and had periodicities of 130-140 nm in the presence of …