[HTML][HTML] Neprilysin degrades both amyloid β peptides 1–40 and 1–42 most rapidly and efficiently among thiorphan-and phosphoramidon-sensitive endopeptidases
K Shirotani, S Tsubuki, N Iwata, Y Takaki… - Journal of Biological …, 2001 - ASBMB
To identify the amyloid β peptide (Aβ) 1–42-degrading enzyme whose activity is inhibited by
thiorphan and phosphoramidon in vivo, we searched for neprilysin (NEP) homologues and
cloned neprilysin-likepeptidase (NEPLP) α, NEPLP β, and NEPLP γ cDNAs. We expressed
NEP, phosphate-regulating gene with homologies to endopeptidases on the X chromosome
(PEX), NEPLPs, and damage-induced neuronal endopeptidase (DINE) in 293 cells as 95-to
125-kDa proteins and found that the enzymatic activities of PEX, NEPLP α, and NEPLP β, as …
thiorphan and phosphoramidon in vivo, we searched for neprilysin (NEP) homologues and
cloned neprilysin-likepeptidase (NEPLP) α, NEPLP β, and NEPLP γ cDNAs. We expressed
NEP, phosphate-regulating gene with homologies to endopeptidases on the X chromosome
(PEX), NEPLPs, and damage-induced neuronal endopeptidase (DINE) in 293 cells as 95-to
125-kDa proteins and found that the enzymatic activities of PEX, NEPLP α, and NEPLP β, as …