Overexpression of the human lysosomal enzyme α-L-iduronidase in Chinese hamster ovary cells
ED Kakkis, A Matynia, AJ Jonas, EF Neufeld - Protein expression and …, 1994 - Elsevier
We developed a Chinese hamster ovary (CHO) cell line that produces and secretes large
quantities of recombinant human α-L-iduronidase, the lysosomal hydrolase deficient in
mucopolysaccharidosis I (Hurler, Hurler-Scheie, and Scheie syndromes). The α-L-
iduronidase cDNA was introduced into a vector containing the cytomegalovirus immediate
early gene promoter/enhancer, a murine immunoglobulin Cα region intron, and the bovine
growth hormone polyadenylation signal. Following cotransfection with a plasmid containing …
quantities of recombinant human α-L-iduronidase, the lysosomal hydrolase deficient in
mucopolysaccharidosis I (Hurler, Hurler-Scheie, and Scheie syndromes). The α-L-
iduronidase cDNA was introduced into a vector containing the cytomegalovirus immediate
early gene promoter/enhancer, a murine immunoglobulin Cα region intron, and the bovine
growth hormone polyadenylation signal. Following cotransfection with a plasmid containing …
