[HTML][HTML] A new determination of the shear modulus of the human erythrocyte membrane using optical tweezers
S Henon, G Lenormand, A Richert, F Gallet - Biophysical journal, 1999 - cell.com
S Henon, G Lenormand, A Richert, F Gallet
Biophysical journal, 1999•cell.comOptical tweezers are used to apply calibrated forces to human erythrocytes, via small silica
beads bound to their membrane. The shear modulus μ of the membrane is inferred from
measurements of the cell deformation in the small strain linear regime. We find the same
result μ= 2.5±0.4μN/m for both discotic and nearly spherical swollen cells. This value is
smaller than the one deduced from micropipettes experiments. However the two methods do
not operate in the same deformation regime and are not expected to lead to the same result.
beads bound to their membrane. The shear modulus μ of the membrane is inferred from
measurements of the cell deformation in the small strain linear regime. We find the same
result μ= 2.5±0.4μN/m for both discotic and nearly spherical swollen cells. This value is
smaller than the one deduced from micropipettes experiments. However the two methods do
not operate in the same deformation regime and are not expected to lead to the same result.
Abstract
Optical tweezers are used to apply calibrated forces to human erythrocytes, via small silica beads bound to their membrane. The shear modulus μ of the membrane is inferred from measurements of the cell deformation in the small strain linear regime. We find the same result μ=2.5±0.4μN/m for both discotic and nearly spherical swollen cells. This value is smaller than the one deduced from micropipettes experiments. However the two methods do not operate in the same deformation regime and are not expected to lead to the same result.
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