[HTML][HTML] Regulation of human B lymphopoiesis by the transforming growth factor-β superfamily in a newly established coculture system using human mesenchymal …
M Ichii, K Oritani, T Yokota, M Nishida… - Experimental …, 2008 - Elsevier
OBJECTIVES: To characterize and evaluate the validity of a novel coculture system for
studying human B-lymphocyte developmental biology. MATERIALS AND METHODS: We
developed a long-term culture system to produce B lymphocytes from human CD34+ cells
purified from umbilical cord blood using human mesenchymal stem cells (hMSC) as stroma.
We evaluated the effects of several low molecular weight inhibitors, recombinant proteins,
and neutralizing antibodies (Abs) as potential regulators of B-lymphocyte development …
studying human B-lymphocyte developmental biology. MATERIALS AND METHODS: We
developed a long-term culture system to produce B lymphocytes from human CD34+ cells
purified from umbilical cord blood using human mesenchymal stem cells (hMSC) as stroma.
We evaluated the effects of several low molecular weight inhibitors, recombinant proteins,
and neutralizing antibodies (Abs) as potential regulators of B-lymphocyte development …
