Expression and characterization of branched-chain α-ketoacid dehydrogenase kinase from the rat: is it a histidine-protein kinase?
The recombinant rat branched-chain α-ketoacid dehydrogenase kinase has been amplified
from rat kidney cDNA, based on the previously reported rat cDNA sequence (Popov, KM,
Zhao, Y., Shimomura, Y., Kuntz, MJ, and Harris, RA (1992) J. Biol. Chem. 267, 13127-
13130). This kinase was expressed in Escherichia coli as a fusion protein with bacterial
maltose-binding protein (MBP). Expression was improved by overexpression of chaperonins
GroEL and GroES. The MBP-kinase, when reconstituted with lipoylated recombinant E2 …
from rat kidney cDNA, based on the previously reported rat cDNA sequence (Popov, KM,
Zhao, Y., Shimomura, Y., Kuntz, MJ, and Harris, RA (1992) J. Biol. Chem. 267, 13127-
13130). This kinase was expressed in Escherichia coli as a fusion protein with bacterial
maltose-binding protein (MBP). Expression was improved by overexpression of chaperonins
GroEL and GroES. The MBP-kinase, when reconstituted with lipoylated recombinant E2 …