[HTML][HTML] Nitric oxide controls src kinase activity through a sulfhydryl group modification-mediated Tyr-527-independent and Tyr-416-linked mechanism
AA Akhand, M Pu, M Kato, H Suzuki, T Senga… - Journal of Biological …, 1999 - ASBMB
c-Src kinase was activated when either murine NIH3T3 fibroblast cells or
immunoprecipitated c-Src proteins were treated with nitric oxide generator, S-nitroso-N-
acetyl penicillamine (SNAP) or sodium nitroprusside. Nitric oxide (NO) scavenger
hemoglobin and N 2 O 3 scavenger homocysteine abolished the SNAP-mediated c-Src
kinase activation. Phosphoamino acid analysis and peptide mapping of in vitro labeled
phospho-c-Src proteins revealed that SNAP promoted the autophosphorylation at tyrosine …
immunoprecipitated c-Src proteins were treated with nitric oxide generator, S-nitroso-N-
acetyl penicillamine (SNAP) or sodium nitroprusside. Nitric oxide (NO) scavenger
hemoglobin and N 2 O 3 scavenger homocysteine abolished the SNAP-mediated c-Src
kinase activation. Phosphoamino acid analysis and peptide mapping of in vitro labeled
phospho-c-Src proteins revealed that SNAP promoted the autophosphorylation at tyrosine …