[HTML][HTML] Differential effects of PAK1-activating mutations reveal activity-dependent and-independent effects on cytoskeletal regulation
PAKs are serine/threonine protein kinases that are activated by binding to Rac or Cdc42hs.
Different forms of activated PAK1 have been reported to either promote membrane ruffling
and focal adhesion assembly or cause focal adhesion disassembly and stress fiber
dissolution. To understand the basis for these distinct morphological effects, we have
examined the mechanism of mutational activation of PAK1, and characterized the effects of
different active PAK1 proteins on cytoskeletal structure in vivo. We find that PAK1 contains …
Different forms of activated PAK1 have been reported to either promote membrane ruffling
and focal adhesion assembly or cause focal adhesion disassembly and stress fiber
dissolution. To understand the basis for these distinct morphological effects, we have
examined the mechanism of mutational activation of PAK1, and characterized the effects of
different active PAK1 proteins on cytoskeletal structure in vivo. We find that PAK1 contains …