Cytosol-stimulated remodeling of phosphatidylcholine in rat lung microsomes
JG Nijssen, H Van den Bosch - Biochimica et Biophysica Acta (BBA)-Lipids …, 1986 - Elsevier
When 600× g supernatants of 10%(w/v) rat lung homogenates were incubated with CDP [Me-
14 C] choline, both saturated and unsaturated species of phosphatidylcholine were formed
from endogenous diacylglycerols. The percentage radioactivity in the disaturated species of
total phosphatidylcholine increased with time from 12% after 5 min to 30% after 60 min
incubation. In similar experiments with 20000× g supernatants, the increase in the
disaturated species of microsomal phosphatidylcholine was from 25 to 37% over the same …
14 C] choline, both saturated and unsaturated species of phosphatidylcholine were formed
from endogenous diacylglycerols. The percentage radioactivity in the disaturated species of
total phosphatidylcholine increased with time from 12% after 5 min to 30% after 60 min
incubation. In similar experiments with 20000× g supernatants, the increase in the
disaturated species of microsomal phosphatidylcholine was from 25 to 37% over the same …