Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution
M Wagner, S Jonjić, UH Koszinowski… - Journal of …, 1999 - Am Soc Microbiol
Journal of virology, 1999•Am Soc Microbiol
Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial
artificial chromosome (BAC)(M. Messerle, I. Crnković, W. Hammerschmidt, H. Ziegler, and
UH Koszinowski, Proc. Natl. Acad. Sci. USA 94: 14759–14763, 1997). The virus obtained
from this construct is attenuated in vivo due to deletion of viral sequences and insertion of
the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector
with identical viral sequences. This new construct represents a versatile basis for …
artificial chromosome (BAC)(M. Messerle, I. Crnković, W. Hammerschmidt, H. Ziegler, and
UH Koszinowski, Proc. Natl. Acad. Sci. USA 94: 14759–14763, 1997). The virus obtained
from this construct is attenuated in vivo due to deletion of viral sequences and insertion of
the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector
with identical viral sequences. This new construct represents a versatile basis for …
Abstract
Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial artificial chromosome (BAC) (M. Messerle, I. Crnković, W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759–14763, 1997). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutants since virus generated from the construct loses the bacterial sequences and acquires wild-type properties.
American Society for Microbiology