Signaling role of intracellular iron in NF-κB activation
S Xiong, H She, H Takeuchi, B Han… - Journal of Biological …, 2003 - ASBMB
Iron chelators inhibit endotoxin-induced NF-κB activation in hepatic macrophages (HMs),
suggesting a role for the intracellular chelatable pool of iron in NF-κB activation. The present
study tested this hypothesis. Analysis of Fe 59-loaded HMs stimulated with
lipopolysaccharide (LPS), revealed a previously unreported, transient rise in intracellular
low molecular weight (LMW)· Fe 59 complex ([LMW· Fe] i) at≤ 2 min returning to the basal
level within 15 min. The [LMW· Fe] i response preceded IκB kinase (IKK)(≥ 15 min) and NF …
suggesting a role for the intracellular chelatable pool of iron in NF-κB activation. The present
study tested this hypothesis. Analysis of Fe 59-loaded HMs stimulated with
lipopolysaccharide (LPS), revealed a previously unreported, transient rise in intracellular
low molecular weight (LMW)· Fe 59 complex ([LMW· Fe] i) at≤ 2 min returning to the basal
level within 15 min. The [LMW· Fe] i response preceded IκB kinase (IKK)(≥ 15 min) and NF …
