[HTML][HTML] Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain
T Reid, T Furuyashiki, T Ishizaki, G Watanabe… - Journal of Biological …, 1996 - ASBMB
Using a mouse embryo cDNA library, we conducted a two-hybrid screening to identify new
partners for the small GTPase Rho. One clone obtained by this procedure contained a novel
cDNA of 291 base pairs and interacted strongly with RhoA and RhoC, weakly with RhoB,
and not at all with Rac1 and Cdc42Hs. Full-length cDNAs were then isolated from a mouse
brain library. While multiple splicing variants were common, we identified three cDNAs with
an identical open reading frame encoding a 61-kDa protein that we named rhotekin (from …
partners for the small GTPase Rho. One clone obtained by this procedure contained a novel
cDNA of 291 base pairs and interacted strongly with RhoA and RhoC, weakly with RhoB,
and not at all with Rac1 and Cdc42Hs. Full-length cDNAs were then isolated from a mouse
brain library. While multiple splicing variants were common, we identified three cDNAs with
an identical open reading frame encoding a 61-kDa protein that we named rhotekin (from …