[HTML][HTML] Identification of the major synaptojanin-binding proteins in brain
E de Heuvel, AW Bell, AR Ramjaun, K Wong… - Journal of Biological …, 1997 - ASBMB
Synaptojanin is a nerve-terminal enriched inositol 5-phosphatase thought to function in
synaptic vesicle endocytosis, in part through interactions with the Src homology 3 domain of
amphiphysin. We have used synaptojanin purified from Sf9 cells after baculovirus mediated
expression in overlay assays to identify two major synaptojanin-binding proteins in rat brain.
The first, at 125 kDa, is amphiphysin. The second, at 40 kDa, is the major synaptojanin-
binding protein detected, is highly enriched in brain, is concentrated in a soluble synaptic …
synaptic vesicle endocytosis, in part through interactions with the Src homology 3 domain of
amphiphysin. We have used synaptojanin purified from Sf9 cells after baculovirus mediated
expression in overlay assays to identify two major synaptojanin-binding proteins in rat brain.
The first, at 125 kDa, is amphiphysin. The second, at 40 kDa, is the major synaptojanin-
binding protein detected, is highly enriched in brain, is concentrated in a soluble synaptic …