[PDF][PDF] CHOLESTEROL SYNTHESIS FROM C14—ACETATE IN MAN
L Hellman, RS Rosenfeld… - The Journal of clinical …, 1954 - Am Soc Clin Investig
L Hellman, RS Rosenfeld, TF Gallagher
The Journal of clinical investigation, 1954•Am Soc Clin InvestigBloch and Rittenberg (1) first demonstrated that acetate could be utilized for cholesterol
svnthesis by animals. In these studies and others (2-4), cholesterol isolated after the
administration of isotopically labeled acetate was shown to contain the administered
isotope. However, there is no information concerning either the dynamic aspects of this
process or the rate of disappearance of labeled cholesterol after its synthesis from ace-tate
in man. 3 In order to study cholesterol synthesis in this manner, radioactive carbon (C14) …
svnthesis by animals. In these studies and others (2-4), cholesterol isolated after the
administration of isotopically labeled acetate was shown to contain the administered
isotope. However, there is no information concerning either the dynamic aspects of this
process or the rate of disappearance of labeled cholesterol after its synthesis from ace-tate
in man. 3 In order to study cholesterol synthesis in this manner, radioactive carbon (C14) …
Bloch and Rittenberg (1) first demonstrated that acetate could be utilized for cholesterol svnthesis by animals. In these studies and others (2-4), cholesterol isolated after the administration of isotopically labeled acetate was shown to contain the administered isotope. However, there is no information concerning either the dynamic aspects of this process or the rate of disappearance of labeled cholesterol after its synthesis from ace-tate in man. 3
In order to study cholesterol synthesis in this manner, radioactive carbon (C14)-labeled acetate was administered to patients, free and ester cholesterol were separately isolated from the plasma, and the radioactivity of these products was measured at frequent intervals over a prolonged period of time.
The Journal of Clinical Investigation