[HTML][HTML] In vivo 13C NMR measurements of hepatocellular tricarboxylic acid cycle flux
BM Jucker, JY Lee, RG Shulman - Journal of Biological Chemistry, 1998 - ASBMB
A combined isotopic steady state and in vivo isotopic non-steady state analysis was used to
calculate tricarboxylic acid cycle flux in livers of anesthetized rats infused with ethanol. In
vivo 13 C NMR spectroscopy was used to non-invasively observe label turnover of [4-13 C]
glutamate,[4-13 C] glutamine, and [2-13 C] glutamate/glutamine in liver following a bolus
intravenous infusion of [2-13 C] ethanol. The isotopic steady state analysis of [2-13 C],[3-13
C], and [4-13 C] glutamate isotopomers (Malloy, CR, Sherry, AD, and Jeffrey, FMH (1988) J …
calculate tricarboxylic acid cycle flux in livers of anesthetized rats infused with ethanol. In
vivo 13 C NMR spectroscopy was used to non-invasively observe label turnover of [4-13 C]
glutamate,[4-13 C] glutamine, and [2-13 C] glutamate/glutamine in liver following a bolus
intravenous infusion of [2-13 C] ethanol. The isotopic steady state analysis of [2-13 C],[3-13
C], and [4-13 C] glutamate isotopomers (Malloy, CR, Sherry, AD, and Jeffrey, FMH (1988) J …