Proteomics investigations typically yield information regarding static gene expression
profiles. The central issues that limit the study of proteome dynamics include how to (i)
administer a labeled amino acid in vivo,(ii) measure the isotopic labeling of a protein
(s)(which may be low), and (iii) reliably interpret the precursor/product labeling relationships.
In this study, we demonstrate the potential of quantifying proteome dynamics by coupling the
administration of stable isotopes with mass spectrometric assays. Although the direct …