Self-organization of human hepatic organoid by recapitulating organogenesis in vitro
BACKGROUND: Careful orchestration among endodermal epithelial, endothelial, and
mesenchymal cells initiate liver organogenesis prior to vascular function. Nonparenchymal
endothelial or mesenchymal cells not only form passive conduits, but also establish an
organogenic stimulus. Herein, we have evaluated the potential roles of primitive endothelial
and mesenchymal cells toward hepatic organization in vitro. METHODS: To track the cellular
movements and localization, we retrovirally transduced enhanced green fluorescence …
mesenchymal cells initiate liver organogenesis prior to vascular function. Nonparenchymal
endothelial or mesenchymal cells not only form passive conduits, but also establish an
organogenic stimulus. Herein, we have evaluated the potential roles of primitive endothelial
and mesenchymal cells toward hepatic organization in vitro. METHODS: To track the cellular
movements and localization, we retrovirally transduced enhanced green fluorescence …