The purpose of this study was to determine whether IP₃Rs contribute to the generation of wide long lasting perinuclear Ca²⁺ release events in canine Purkinje cells. Spontaneous Ca²⁺ release events (elevations of basal [Ca²⁺] equivalent to F/F₀ 3.4SD over F₀) were imaged using Fluo-4AM and 2D confocal microscope. Only cells free of Ca²⁺ waves were analyzed. Subsarcolemmal region (SSL) was defined as 5 μm from cell edges. Core was the remaining cell. The majority of events (94%, 0.0035±0.0007 events (ev)/μm²/s, n=34 cells) were detected within a single frame (typical events, TE). However, a subpopulation (6.0%, 0.00022±0.00005 ev/μm²/s, n=41 cells: wide long lasting events, WLE) lasted for several frames, showed a greater spatial extent (51.0±3.9 vs. TE 9.0±0.3 μm², P<0.01) and higher amplitude (F/F₀ 1.38±0.02 vs. TE 1.20±0.003, P<0.01). WLE event rate was increased by phenylephrine (10 μM, P<0.01), inhibited by 2APB and U73122 (P<0.05), and abolished by tetracaine (1 mM) and ryanodine (100 μM). While SSL WLEs were scattered randomly, Core WLEs (n=(n69 events) were predominantly distributed longitudinally 18.2±1.6 μm from the center of nuclei. Immunocytochemistry showed that IP₃R1s were located not only at SSL region but also near both ends of nucleus overlapping with RyRs. In Purkinje cells, wide long lasting Ca²⁺ release events occur in SSL and in specific perinuclear regions. They are likely due to RyRs and IP₃R1s evoked Ca²⁺ release and may play a role in Ca²⁺ dependent nuclear processes.