A genetically defined normal human somatic cell system to study ras oncogenesis in vivo and in vitro
KM O'Hayer, CM Counter - Methods in enzymology, 2006 - Elsevier
Transgenic mice, cultured murine cells, and human cancer cell lines have widely been used
to study Ras oncogenesis. Although extremely valuable systems, they could not be used to
study Ras function in genetically defined human cells. In this regard, Ras is required for
tumor formation in normal human somatic cells expressing SV‐40 T/t antigens, which
inactivate the tumor suppressors p53 and Rb and activate the oncogene c‐Myc, and hTERT,
the catalytic subunit of telomerase. Such a system allows not only the general requirements …
to study Ras oncogenesis. Although extremely valuable systems, they could not be used to
study Ras function in genetically defined human cells. In this regard, Ras is required for
tumor formation in normal human somatic cells expressing SV‐40 T/t antigens, which
inactivate the tumor suppressors p53 and Rb and activate the oncogene c‐Myc, and hTERT,
the catalytic subunit of telomerase. Such a system allows not only the general requirements …
