[HTML][HTML] Novel genes upregulated when NOTCH signalling is disrupted during hypothalamic development
Neural development, 2013•Springer
Background The generation of diverse neuronal types and subtypes from multipotent
progenitors during development is crucial for assembling functional neural circuits in the
adult central nervous system. It is well known that the Notch signalling pathway through the
inhibition of proneural genes is a key regulator of neurogenesis in the vertebrate central
nervous system. However, the role of Notch during hypothalamus formation along with its
downstream effectors remains poorly defined. Results Here, we have transiently blocked …
progenitors during development is crucial for assembling functional neural circuits in the
adult central nervous system. It is well known that the Notch signalling pathway through the
inhibition of proneural genes is a key regulator of neurogenesis in the vertebrate central
nervous system. However, the role of Notch during hypothalamus formation along with its
downstream effectors remains poorly defined. Results Here, we have transiently blocked …
Background
The generation of diverse neuronal types and subtypes from multipotent progenitors during development is crucial for assembling functional neural circuits in the adult central nervous system. It is well known that the Notch signalling pathway through the inhibition of proneural genes is a key regulator of neurogenesis in the vertebrate central nervous system. However, the role of Notch during hypothalamus formation along with its downstream effectors remains poorly defined.
Results
Here, we have transiently blocked Notch activity in chick embryos and used global gene expression analysis to provide evidence that Notch signalling modulates the generation of neurons in the early developing hypothalamus by lateral inhibition. Most importantly, we have taken advantage of this model to identify novel targets of Notch signalling, such as Tagln3 and Chga, which were expressed in hypothalamic neuronal nuclei.
Conclusions
These data give essential advances into the early generation of neurons in the hypothalamus. We demonstrate that inhibition of Notch signalling during early development of the hypothalamus enhances expression of several new markers. These genes must be considered as important new targets of the Notch/proneural network.
Springer