[HTML][HTML] Evidence against defective trans-Golgi acidification in cystic fibrosis
O Seksek, J Biwersi, AS Verkman - Journal of Biological Chemistry, 1996 - ASBMB
Defective organelle acidification has been proposed as a unifying hypothesis to explain the
pleiotropic cellular abnormalities associated with cystic fibrosis. To test whether cystic
fibrosis transmembrane conductance regulator (CFTR) participates in trans-Golgi pH
regulation, intraluminal trans-Golgi pH was measured in stably transfected Swiss 3T3
fibroblasts (expressing CFTR or ΔF508-CFTR) and CFTR-expressing and nonexpressing
epithelial cells. trans-Golgi pH was measured by ratio-imaging confocal microscopy using a …
pleiotropic cellular abnormalities associated with cystic fibrosis. To test whether cystic
fibrosis transmembrane conductance regulator (CFTR) participates in trans-Golgi pH
regulation, intraluminal trans-Golgi pH was measured in stably transfected Swiss 3T3
fibroblasts (expressing CFTR or ΔF508-CFTR) and CFTR-expressing and nonexpressing
epithelial cells. trans-Golgi pH was measured by ratio-imaging confocal microscopy using a …