Structural basis for the positional specificity of lipoxygenases
H Kuhn - Prostaglandins & other lipid mediators, 2000 - Elsevier
The positional specificity of arachidonic acid oxygenation is currently the decisive parameter
for classification of mammalian lipoxygenases but, unfortunately, the structural reasons for
lipoxygenase specificity are not well understood. Although there are no direct structural data
on lipoxygenase/substrate interaction, experiments with modified fatty acid substrates and
mutagenesis studies suggest that for 12-and 15-lipoxygenases, arachidonic acid slides into
the substrate-binding pocket with its methyl end ahead. For arachidonate 5-and/or 8 …
for classification of mammalian lipoxygenases but, unfortunately, the structural reasons for
lipoxygenase specificity are not well understood. Although there are no direct structural data
on lipoxygenase/substrate interaction, experiments with modified fatty acid substrates and
mutagenesis studies suggest that for 12-and 15-lipoxygenases, arachidonic acid slides into
the substrate-binding pocket with its methyl end ahead. For arachidonate 5-and/or 8 …