Proteome-wide measurement of protein half-lives and translation rates in vasopressin-sensitive collecting duct cells
PC Sandoval, DH Slentz, T Pisitkun… - Journal of the …, 2013 - journals.lww.com
Vasopressin regulates water excretion, in part, by controlling the abundances of the water
channel aquaporin-2 (AQP2) protein and regulatory proteins in the renal collecting duct. To
determine whether vasopressin-induced alterations in protein abundance result from
modulation of protein production, protein degradation, or both, we used protein mass
spectrometry with dynamic stable isotope labeling in cell culture to achieve a proteome-wide
determination of protein half-lives and relative translation rates in mpkCCD cells …
channel aquaporin-2 (AQP2) protein and regulatory proteins in the renal collecting duct. To
determine whether vasopressin-induced alterations in protein abundance result from
modulation of protein production, protein degradation, or both, we used protein mass
spectrometry with dynamic stable isotope labeling in cell culture to achieve a proteome-wide
determination of protein half-lives and relative translation rates in mpkCCD cells …