Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex

M Hamady, JJ Walker, JK Harris, NJ Gold, R Knight - Nature methods, 2008 - nature.com
M Hamady, JJ Walker, JK Harris, NJ Gold, R Knight
Nature methods, 2008nature.com
We constructed error-correcting DNA barcodes that allow one run of a massively parallel
pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we
processed bacterial 16S rRNA gene sequences representing microbial communities in 286
environmental samples, corrected 92% of sample assignment errors, and thus characterized
nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
Abstract
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
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