Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex
We constructed error-correcting DNA barcodes that allow one run of a massively parallel
pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we
processed bacterial 16S rRNA gene sequences representing microbial communities in 286
environmental samples, corrected 92% of sample assignment errors, and thus characterized
nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we
processed bacterial 16S rRNA gene sequences representing microbial communities in 286
environmental samples, corrected 92% of sample assignment errors, and thus characterized
nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
Abstract
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
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