IL-13Rα2/AP-1 complex signalling mediates airway epithelial repair without effects on remodeling pathways

JS Yang, S Allahverdian, GK Singhera… - Allergy, Asthma & …, 2010 - Springer
JS Yang, S Allahverdian, GK Singhera, RE MacRedmond, DR Dorscheid
Allergy, Asthma & Clinical Immunology, 2010Springer
Methods IL-13Rα1 and IL-13Rα2 functions were disrupted in Human Airway Epithelial
(1HAEo-) cells using specific IL-13Rα1 and IL-13Rα2 blocking antibodies and small
interfering RNAs (siRNAs). 1HAEo-cells were also transfected with activator protein 1 (AP-1)
specific and scramble siRNA. Following specific antibody blocking or siRNA transfection,
1HAEo-cells were either stimulated with IL-13 (10 ng/ml) or mechanically injured.
Supernatants and protein lysates were collected at different time points. Expressions of …
Methods
IL-13Rα1 and IL-13Rα2 functions were disrupted in Human Airway Epithelial (1HAEo-) cells using specific IL-13Rα1 and IL-13Rα2 blocking antibodies and small interfering RNAs (siRNAs). 1HAEo-cells were also transfected with activator protein 1 (AP-1) specific and scramble siRNA. Following specific antibody blocking or siRNA transfection, 1HAEo-cells were either stimulated with IL-13 (10 ng/ml) or mechanically injured. Supernatants and protein lysates were collected at different time points. Expressions of phospho-STAT6, STAT6, Egr1, and AP-1 were detected via Western blotting, while HB-EGF release in supernatants was quantified using ELISA. Furthermore, AP-1 activity in 1HAEo-cells after IL-13 stimulation or mechanical injury was measured using an AP-1-luciferase assay.
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