Hypoxia Leads to Na,K-ATPase Downregulation via Ca2+ Release-Activated Ca2+ Channels and AMPK Activation

GA Gusarova, HE Trejo, LA Dada, A Briva… - … and cellular biology, 2011 - Taylor & Francis
GA Gusarova, HE Trejo, LA Dada, A Briva, LC Welch, RB Hamanaka, GM Mutlu
Molecular and cellular biology, 2011Taylor & Francis
To maintain cellular ATP levels, hypoxia leads to Na, K-ATPase inhibition in a process
dependent on reactive oxygen species (ROS) and the activation of AMP-activated kinase α1
(AMPK-α1). We report here that during hypoxia AMPK activation does not require the liver
kinase B1 (LKB1) but requires the release of Ca2+ from the endoplasmic reticulum (ER) and
redistribution of STIM1 to ER-plasma membrane junctions, leading to calcium entry via Ca2+
release-activated Ca2+ (CRAC) channels. This increase in intracellular Ca2+ induces …
To maintain cellular ATP levels, hypoxia leads to Na,K-ATPase inhibition in a process dependent on reactive oxygen species (ROS) and the activation of AMP-activated kinase α1 (AMPK-α1). We report here that during hypoxia AMPK activation does not require the liver kinase B1 (LKB1) but requires the release of Ca2+ from the endoplasmic reticulum (ER) and redistribution of STIM1 to ER-plasma membrane junctions, leading to calcium entry via Ca2+ release-activated Ca2+ (CRAC) channels. This increase in intracellular Ca2+ induces Ca2+/calmodulin-dependent kinase kinase β (CaMKKβ)-mediated AMPK activation and Na,K-ATPase downregulation. Also, in cells unable to generate mitochondrial ROS, hypoxia failed to increase intracellular Ca2+ concentration while a STIM1 mutant rescued the AMPK activation, suggesting that ROS act upstream of Ca2+ signaling. Furthermore, inhibition of CRAC channel function in rat lungs prevented the impairment of alveolar fluid reabsorption caused by hypoxia. These data suggest that during hypoxia, calcium entry via CRAC channels leads to AMPK activation, Na,K-ATPase downregulation, and alveolar epithelial dysfunction.
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