Real-time imaging of axonal transport of quantum dot-labeled BDNF in primary neurons
X Zhao, Y Zhou, AM Weissmiller, ML Pearn… - JoVE (Journal of …, 2014 - jove.com
BDNF plays an important role in several facets of neuronal survival, differentiation, and
function. Structural and functional deficits in axons are increasingly viewed as an early
feature of neurodegenerative diseases, including Alzheimer's disease (AD) and
Huntington's disease (HD). As yet unclear is the mechanism (s) by which axonal injury is
induced. We reported the development of a novel technique to produce biologically active,
monobiotinylated BDNF (mBtBDNF) that can be used to trace axonal transport of BDNF …
function. Structural and functional deficits in axons are increasingly viewed as an early
feature of neurodegenerative diseases, including Alzheimer's disease (AD) and
Huntington's disease (HD). As yet unclear is the mechanism (s) by which axonal injury is
induced. We reported the development of a novel technique to produce biologically active,
monobiotinylated BDNF (mBtBDNF) that can be used to trace axonal transport of BDNF …