Translating Sleeping Beauty transposition into cellular therapies: Victories and challenges

Z Izsvák, PB Hackett, LJN Cooper, Z Ivics - Bioessays, 2010 - Wiley Online Library
Bioessays, 2010Wiley Online Library
Recent results confirm that long‐term expression of therapeutic transgenes can be achieved
by using a transposon‐based system in primary stem cells and in vivo. Transposable
elements are natural DNA transfer vehicles that are capable of efficient genomic insertion.
The latest generation, Sleeping Beauty transposon‐based hyperactive vector (SB100X), is
able to address the basic problem of non‐viral approaches–that is, low efficiency of stable
gene transfer. The combination of transposon‐based non‐viral gene transfer with the latest …
Abstract
Recent results confirm that long‐term expression of therapeutic transgenes can be achieved by using a transposon‐based system in primary stem cells and in vivo. Transposable elements are natural DNA transfer vehicles that are capable of efficient genomic insertion. The latest generation, Sleeping Beauty transposon‐based hyperactive vector (SB100X), is able to address the basic problem of non‐viral approaches – that is, low efficiency of stable gene transfer. The combination of transposon‐based non‐viral gene transfer with the latest improvements of non‐viral delivery techniques could provide a long‐term therapeutic effect without compromising biosafety. The new challenges of pre‐clinical research will focus on further refinement of the technology in large animal models and improving the safety profile of SB vectors by target‐selected transgene integration into genomic “safe harbors.” The first clinical application of the SB system will help to validate the safety of this approach.
Wiley Online Library