Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells

J Jozefczuk, K Drews, J Adjaye - JoVE (Journal of Visualized Experiments), 2012 - jove.com
In general, human embryonic stem cells (hESCs) and human induced pluripotent stem cells
(hiPSCs) 1 can be cultured under variable conditions. However, it is not easy to establish an
effective system for culturing these cells. Since the culture conditions can influence gene
expression that confers pluripotency in hESCs and hiPSCs, the optimization and
standardization of the culture method is crucial. The establishment of hESC lines was first
described by using MEFs as feeder cells and fetal bovine serum (FBS)-containing culture …