Optogenetic control of heart muscle in vitro and in vivo

T Bruegmann, D Malan, M Hesse, T Beiert… - Nature …, 2010 - nature.com
T Bruegmann, D Malan, M Hesse, T Beiert, CJ Fuegemann, BK Fleischmann, P Sasse
Nature methods, 2010nature.com
Electrical stimulation is the standard technique for exploring electrical behavior of heart
muscle, but this approach has considerable technical limitations. Here we report expression
of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of
heart muscle in vitro and in mice. This method enabled precise localized stimulation and
constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in
alterations of pacemaking, Ca2+ homeostasis, electrical coupling and arrhythmogenic …
Abstract
Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca2+ homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.
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