The study of electrophysiological properties of cardiac ion channels with the patch-clamp
technique and the exploration of cardiac cellular Ca2+ handling abnormalities requires
isolated cardiomyocytes. In addition, the possibility to investigate myocytes from patients
using these techniques is an invaluable requirement to elucidate the molecular basis of
cardiac diseases such as atrial fibrillation (AF). 1 Here we describe a method for isolation of
human atrial myocytes which are suitable for both patch-clamp studies and simultaneous …