[HTML][HTML] Mass and positional isotopomer analysis of glucose metabolism in periportal and pericentral hepatocytes
GW Cline, GI Shulman - Journal of Biological Chemistry, 1995 - ASBMB
To determine whether the source of carbon for the indirect pathway of hepatic glycogen
synthesis differs between the periportal and pericentral zones, we studied seven 24-h-fasted
conscious rats given a constant 2-h intraduodenal infusion of glucose, 40% labeled with [U-
13 C] glucose (99% 13 C enriched), to raise and maintain plasma glucose concentration at~
10 mM. Glycogen, glutamate, aspartate, and alanine were selectively sampled from the
periportal and pericentral zones of the liver by the dual-digitonin pulse technique and …
synthesis differs between the periportal and pericentral zones, we studied seven 24-h-fasted
conscious rats given a constant 2-h intraduodenal infusion of glucose, 40% labeled with [U-
13 C] glucose (99% 13 C enriched), to raise and maintain plasma glucose concentration at~
10 mM. Glycogen, glutamate, aspartate, and alanine were selectively sampled from the
periportal and pericentral zones of the liver by the dual-digitonin pulse technique and …