The origin and development of nonlymphoid tissue CD103+ DCs

F Ginhoux, K Liu, J Helft, M Bogunovic… - Journal of Experimental …, 2009 - rupress.org
F Ginhoux, K Liu, J Helft, M Bogunovic, M Greter, D Hashimoto, J Price, N Yin, J Bromberg…
Journal of Experimental Medicine, 2009rupress.org
CD103+ dendritic cells (DCs) in nonlymphoid tissues are specialized in the cross-
presentation of cell-associated antigens. However, little is known about the mechanisms that
regulate the development of these cells. We show that two populations of CD11c+ MHCII+
cells separated on the basis of CD103 and CD11b expression coexist in most nonlymphoid
tissues with the exception of the lamina propria. CD103+ DCs are related to lymphoid organ
CD8+ DCs in that they are derived exclusively from pre-DCs under the control of fms-like …
CD103+ dendritic cells (DCs) in nonlymphoid tissues are specialized in the cross-presentation of cell-associated antigens. However, little is known about the mechanisms that regulate the development of these cells. We show that two populations of CD11c+MHCII+ cells separated on the basis of CD103 and CD11b expression coexist in most nonlymphoid tissues with the exception of the lamina propria. CD103+ DCs are related to lymphoid organ CD8+ DCs in that they are derived exclusively from pre-DCs under the control of fms-like tyrosine kinase 3 (Flt3) ligand, inhibitor of DNA protein 2 (Id2), and IFN regulatory protein 8 (IRF8). In contrast, lamina propria CD103+ DCs express CD11b and develop independently of Id2 and IRF8. The other population of CD11c+MHCII+ cells in tissues, which is CD103CD11b+, is heterogenous and depends on both Flt3 and MCSF-R. Our results reveal that nonlymphoid tissue CD103+ DCs and lymphoid organ CD8+ DCs derive from the same precursor and follow a related differentiation program.
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