BACKGROUND: Loss-of-function mutations in filaggrin are major predisposing factors for
atopic dermatitis. Although various reports suggest a critical role for filaggrin in stratum
corneum (SC) barrier formation, the lack of filaggrin-null (Flg−/−) mice has hampered
detailed in vivo analysis of filaggrin's functions. OBJECTIVE: We sought to generate Flg−/−
mice and to assess the effect of filaggrin loss on SC barrier function and percutaneous
immune responses. METHODS: We generated Flg−/− mice using gene targeting and …