[HTML][HTML] Large-scale targeted proteomics using internal standard triggered-parallel reaction monitoring (IS-PRM)*[S]
S Gallien, SY Kim, B Domon - Molecular & Cellular Proteomics, 2015 - ASBMB
Targeted high-resolution and accurate mass analyses performed on fast sequencing mass
spectrometers have opened new avenues for quantitative proteomics. More specifically,
parallel reaction monitoring (PRM) implemented on quadrupole-orbitrap instruments
exhibits exquisite selectivity to discriminate interferences from analytes. Furthermore, the
instrument trapping capability enhances the sensitivity of the measurements. The PRM
technique, applied to the analysis of limited peptide sets (typically 50 peptides or less) in a …
spectrometers have opened new avenues for quantitative proteomics. More specifically,
parallel reaction monitoring (PRM) implemented on quadrupole-orbitrap instruments
exhibits exquisite selectivity to discriminate interferences from analytes. Furthermore, the
instrument trapping capability enhances the sensitivity of the measurements. The PRM
technique, applied to the analysis of limited peptide sets (typically 50 peptides or less) in a …