GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution

MA Puthenveedu, C Bachert, S Puri, F Lanni… - Nature cell …, 2006 - nature.com
MA Puthenveedu, C Bachert, S Puri, F Lanni, AD Linstedt
Nature cell biology, 2006nature.com
The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form
a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose
of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is
mediated by specific membrane-fusion events that occur during Golgi assembly, and require
the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-
dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of …
Abstract
The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of enzymes in the Golgi ribbon. The membrane continuity created by ribbon formation facilitates optimal processing conditions in the biosynthetic pathway.
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