[HTML][HTML] Mapping the functional domains of the Golgi stacking factor GRASP65

Y Wang, A Satoh, G Warren - Journal of Biological Chemistry, 2005 - ASBMB
The Golgi reassembly stacking protein (GRASP) family has been implicated in the stacking
of Golgi cisternae and the regulation of Golgi disassembly/reassembly during mitosis in
mammalian cells. GRASP65 is a dimer that can directly link adjacent surfaces through trans-
oligomerization in a mitotically regulated manner. Here we show that the N-terminal GRASP
domain (amino acids 1–201) is both necessary and sufficient for dimerization and trans-
oligomerization but is not mitotically regulated. The C-terminal serine/proline-rich domain …