Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans
A skin blister technique is described which allows the investigation of acute inflammation in
humans in vivo. Filter paper discs are placed on the skin, impregnated with cantharidin and
covered with impermeable film held by adhesive tape. The assembly is easily applied,
unobtrusive, stable and may be worn during normal activities. The blister formed at 24 h
contains approximately 5× 105–5× 106 cells, predominantly neutrophils and macrophages.
Inflammatory cytokines and chemotactic factors are detectable in the blister fluid. The …
humans in vivo. Filter paper discs are placed on the skin, impregnated with cantharidin and
covered with impermeable film held by adhesive tape. The assembly is easily applied,
unobtrusive, stable and may be worn during normal activities. The blister formed at 24 h
contains approximately 5× 105–5× 106 cells, predominantly neutrophils and macrophages.
Inflammatory cytokines and chemotactic factors are detectable in the blister fluid. The …