[HTML][HTML] Identifying key residues of sphinganine-1-phosphate lyase for function in vivo and in vitro

D Mukhopadhyay, KS Howell, H Riezman… - Journal of biological …, 2008 - ASBMB
Sphinganine-1-phosphate lyase (Dpl1p) is a highly conserved enzyme of sphingolipid
metabolism that catalyzes the irreversible degradation of sphingoid base phosphates, which
are potent signaling molecules. Sphingoid base phosphates play a vital role in cell survival,
proliferation, migration, heat stress, and cell wall integrity pathways. Little is known about the
structure and regulation of Dpl1p. In this study, we have undertaken a combined
computational modeling and mutagenesis approach for structure-function analysis of Dpl1p …