[HTML][HTML] RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1
S Mezzar, E De Schryver, PP Van Veldhoven - Journal of lipid research, 2014 - ASBMB
Long-chain aldehydes are commonly produced in various processes, such as peroxisomal α-
oxidation of long-chain 3-methyl-branched and 2-hydroxy fatty acids and microsomal
breakdown of phosphorylated sphingoid bases. The enzymes involved in the aldehyde-
generating steps of these processes are 2-hydroxyacyl-CoA lyase (HACL1) and
sphingosine-1-phosphate lyase (SGPL1), respectively. In the present work, nonradioactive
assays for these enzymes were developed employing the Hantzsch reaction. Tridecanal …
oxidation of long-chain 3-methyl-branched and 2-hydroxy fatty acids and microsomal
breakdown of phosphorylated sphingoid bases. The enzymes involved in the aldehyde-
generating steps of these processes are 2-hydroxyacyl-CoA lyase (HACL1) and
sphingosine-1-phosphate lyase (SGPL1), respectively. In the present work, nonradioactive
assays for these enzymes were developed employing the Hantzsch reaction. Tridecanal …