[HTML][HTML] Structure of complement C3 (H2O) revealed by quantitative cross-linking/mass spectrometry and modeling

ZA Chen, R Pellarin, L Fischer, A Sali, M Nilges… - Molecular & Cellular …, 2016 - ASBMB
The slow but spontaneous and ubiquitous formation of C3 (H 2 O), the hydrolytic and
conformationally rearranged product of C3, initiates antibody-independent activation of the
complement system that is a key first line of antimicrobial defense. The structure of C3 (H 2
O) has not been determined. Here we subjected C3 (H 2 O) to quantitative cross-
linking/mass spectrometry (QCLMS). This revealed details of the structural differences and
similarities between C3 (H 2 O) and C3, as well as between C3 (H 2 O) and its pivotal …