Enhanced chemiluminescence (ECL) for routine immunoblotting: An inexpensive alternative to commercially available kits

DD Mruk, CY Cheng - Spermatogenesis, 2011 - Taylor & Francis
DD Mruk, CY Cheng
Spermatogenesis, 2011Taylor & Francis
Immunoblotting is an analytical technique used by many laboratories to study protein
expression. It involves electrophoretic separation of proteins by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE), immobilization of these proteins onto a
membrane (ie, nitrocellulose or polyvinylidene difluoride), incubation in a monoclonal or
polyclonal antibody and detection by a standard method such as enhanced
chemiluminescence (ECL). To achieve this, most laboratories opt to use commercially …
Immunoblotting is an analytical technique used by many laboratories to study protein expression. It involves electrophoretic separation of proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), immobilization of these proteins onto a membrane (i.e., nitrocellulose or polyvinylidene difluoride), incubation in a monoclonal or polyclonal antibody and detection by a standard method such as enhanced chemiluminescence (ECL). To achieve this, most laboratories opt to use commercially-available chemiluminescence kits which are acceptable but relatively expensive. In this technical report, we show that a self-prepared chemiluminescence reagent is superior to a commercially obtained kit in terms of sensitivity, duration of signal, ease-of-use and shelf-life but at a fraction of the cost of a kit.
Taylor & Francis Online