[HTML][HTML] DDRGK1 regulates NF-κB activity by modulating IκBα stability

P Xi, D Ding, J Zhou, M Wang, YS Cong - PLoS One, 2013 - journals.plos.org
P Xi, D Ding, J Zhou, M Wang, YS Cong
PLoS One, 2013journals.plos.org
NF-κB is a ubiquitously expressed transcription factor that regulates a large number of
genes in response to diverse physiological and pathological stimuli. The regulation of the
transcriptional activity of NF-κB is often dependent on its interaction with IκBα. Proteins that
bind to IκBα are critical regulators of NF-κB activity. DDRGK1 is a member of the DDRGK
domain-containing protein family with unknown function. In this study, we showed that the
depletion of DDRGK1 inhibits cell proliferation and invasion. Microarray analysis indicated …
NF-κB is a ubiquitously expressed transcription factor that regulates a large number of genes in response to diverse physiological and pathological stimuli. The regulation of the transcriptional activity of NF-κB is often dependent on its interaction with IκBα. Proteins that bind to IκBα are critical regulators of NF-κB activity. DDRGK1 is a member of the DDRGK domain-containing protein family with unknown function. In this study, we showed that the depletion of DDRGK1 inhibits cell proliferation and invasion. Microarray analysis indicated that the expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. We further demonstrated that DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. Our findings identify DDRGK1 as an important regulator of the NF-κB pathway.
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